SOURSOP (ANNONA MURICATA) ANTICANCER AND HERBAL PROPERTIES !
Graviola: A novel promising natural-derived drug that inhibits tumorigenicity and metastasis of pancreatic cancer cells in vitro and in vivo through altering cell metabolism by :
María P. Torresa, b, Satyanarayana Rachagania, Vinee Purohitb, Poomy Pandeyc, Suhasini Joshia, Erik D. Moorea, Sonny L. Johanssonb, d, Pankaj K. Singha, b, Apar K. Gantie, Surinder K. Batraa, b, d, ,
a Department of Biochemistry and Molecular Biology, Omaha, NE 68198-5870, USA
b Eppley Institute for Research in Cancer and Allied Diseases, Omaha, NE 68198-5870, USA
c Department of Environmental, Agricultural & Occupational Health, Omaha, NE 68198-5870, USA
d Department of Pathology and Microbiology, Omaha, NE 68198-5870, USA
e Department of Internal Medicine VA Nebraska-Western Iowa Health Care System and University of Nebraska Medical Center, Omaha, NE 68198-5870, USA
The following is a review on a paper I read up on titled : Graviola: A novel promising natural-derived drug that inhibits tumorigenicity and metastasis of pancreatic cancer cells in vitro and in vivo through altering cell metabolism. Various parts of the Graviola tree — including the bark, leaves, roots, fruit and fruit-seeds — have been used for centuries by medicine men and native Indians in South America to treat heart disease, asthma, liver problems and arthritis.
In this paper they show that the Pancreatic tumors are resilient to chemotherapies utilised in cancer normally for treatment . the aim of the study illustrated research done to determine the possibility of a innovative plant-made product or substance that can be utilised as a healing agent for pancreatic cancer . in the study the direct effects of an Annona Muricata (soursop ) also known as graviola , was assessed and tested for the following :cytotoxicity, cell metabolism, cancer-associated protein/gene expression, tumorigenicity, and metastatic properties of the cancerous cells.
Studies from 1998 to 2000 by McLaughlin and Chih HW, Chui HF have shown that acetogenins are inhibitors of complex I of oxidative phosphorylation chain thereby block the formation of ATP energy needed by the cancer cell to operate your pump P-glycoprotein mediated, allowing you to stay active. The acetogenins also inhibit the ubiquinone-ubiquinone oxidase, NADH-dependent enzyme that is peculiar to the plasma membrane of the cancer cell.
On completion of the research data obtained form the experiments that the soursop (annona murciata ) promoted and subsequently caused necrosis of the pancreatic cancer cells by the process of cellular metabolism inhibition .
Their research Overall, the compounds that are naturally present in a Graviola extract inhibited multiple signaling pathways that regulate metabolism, cell cycle, survival, and metastatic properties in PC cells. Collectively, alterations in these parameters led to a decrease in tumorigenicity and metastasis of orthotopically implanted pancreatic tumors, indicating promising characteristics of the natural product against this lethal disease.
Their research illustrated that basically all the antioxidants and bioactive compounds that are present in the soursop fruit extract resulted in inhibition several signalling pathways that were responsible for regulating pathways for cell cycle metabolism , its continued existence and also its ability to metastasize in the cancerous cells . Also changes in these specific parameters resulted in a significant decrease in the tumours mestasizing or growing . Thus demonstrating favourable characteristics of the annona muricata aka soursop against cancers .
Knowledge about an increased consumption of fruits and vegetables is associated with a reduced risk of most cancers, including PC is popular and known worldwide . The major bioactive components that have been extracted from different parts of the plant are known as Annonaceous acetogenins.
For the research conducted for the study a series of tests and experiments were conducted such as : testing the graviola extract first . the cytoxicity and antibodies were tested as well . western blot analysis and real time PCR were used to test the samples . glucose uptake and ATP quantification and cell cycle ananlysis were done on the data . then In vivo tumorigenicity studies conducted on samples displayed positive results for soursops anticancer properties .
The research paper goes on to state that according to the obtained results from in vitro experiments, upon statistical analysis the ‘ Graviola extract has promising properties to be incorporated in PC therapeutics.’ However, these anti-tumorigenic properties entail advance validation through in vivo experiments.
The present study is the first to demonstrate that Graviola extract reduces the viability of PC cells and tumors by inducing necrosis and cell cycle arrest, and by inhibiting PC cell motility (i.e. cytoskeleton rearrangement), migration, and metabolism. Overall, in vitro experiments revealed that the compounds present in the natural extract inhibited several pathways involved in PC cell proliferation and metabolism, simultaneously. Such inhibitions ultimately led to a decrease in tumor growth and metastasis in orthotopically transplanted pancreatic tumor-bearing mice
They stated that their study was in fact the first of its like to show that soursop (graviola) extracts in fact decreases the pancreatic cancerous cells sustainability by the process of inducing cell cycle arrest and necrosis by the cancerous tumor cell motility inhibition . they data show that these inhibitions in the end steered to tumor growth decreasing and metastasis in orthotopically transplanted pancreatic tumor-bearing mice.
While necrotic agents have not been considered beneficial in cancer therapies due to induction of local inflammation, the process itself can lead to the activation of the innate immune system capable of initiating anti-tumor immunity ( R.K. Amaravadi, C.B. Thompson)
The main bioactive compounds recognised in Annona Muricata(soursop) have been classified as Annonaceous acetogenins, which causes the inhibition of mitochondrial complex I that consequently results in ATP production being decreased . Despite the fact that the extract capsules utilised in this research had many other compounds within it .The Annonaceous acetogenins were clearly evident of being present and illustrated a significant depletion of the ATP production in the pancreatic cancer cells upon being treated with the graviola extract
- The roles of therapy-induced autophagy and necrosis in cancer treatment by R.K. Amaravadi, C.B. Thompson.